auto PAF-AH Assay

(Platelet activating factor acetylhydrolase assay)

Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of mammalian celltypes. It is a mediator for allergic and inflammatory reactions. PAF is converted to the biologically inactive lyso-PAF by PAF acetylhydrolase (PAF-AH), which catalyzes the hydrolysis of the esterified acetate at the sn-2 position. PAF-AH also degrades oxidized phospholipids that are structurally similar to PAF and closely associated with atherosclerosis.

These observations suggest that PAF-AH plays an important roll in allergic, inflammatory, and atherosclerotic diseases. Changes in human plasma or serum PAF-AH activity have been observed in a variety of diseases. Auto PAF-AH is an accurate and convenient assay for the measurement of serum (plasma) PAF-AH.

The assay uses a spectrophotometric method, and does not require any pretreatment of samples, or use any radioisotopes. In conjunction with an automatic analyzer, it is possible to measure the activities of thousands of samples in a few hours. Consisting of liquid reagents , the kit is stable for 11 months at 2–8 ·C. Upon preparation of the buffer solution the kit is stable for 14 days. The assay is linear up to 1500 IU/L, and is unaffected by hemoglobin, bilirubin, or chylomicron, as well as a variety of esterases.The kit is designed for 500 tests.

Catalog # PAF7413
Product Insert